Posted by PeterJ on April 26, 2000, at 2:51:40
In reply to Investigational drug assay and specific receptors., posted by Dr. Fried on April 25, 2000, at 23:15:44
> Can anyone tell me how they determine the activity of an investigational drug at specific receptors, (5HT1a,5HT2a, etc., etc,), before human trials? What experimental model do they use, (particular tissue etc.)?
"Bind and grind" studies, they are colloquially called. Many techniques are in use.
One common method is to use suspensions of membranes from cell lines
which have been transfected with cloned receptor DNA to cause them
to express specific receptor types.Common cell lines in use are:
CHO: Chinese Hamster Ovary
HEK: Human Embryonic Kidney
Sf9: Spodoptera Frugiperda (fall army worm) pupal ovaryYou can purchase various serotonin receptor preparations
using these cell lines, for example 5-HT1a receptors
in Sf9 cells.Here is the URL of a typical company (one of many) which sells such
cell lines:http://www.receptorbiology.com/
(I don't have any financial interest in that company.)
Peter
poster:PeterJ
thread:31294
URL: http://www.dr-bob.org/babble/20000420/msgs/31311.html