Posted by tealady on August 27, 2003, at 8:49:31
In reply to Re: Receive Mail-In Hormone test from home » jparsell82, posted by BarbaraCat on August 26, 2003, at 12:23:25
OK, I looked up some research ..on saliva versus blood testing in progesterone and estradiol...difficult to say.
I have heard from many girls that long term progesterone cream does have an effect..so much so that most stop after a year from too much (if they weren't cycling it)...all I can guess from this is the researchers didn't use a very good cream?? or perhaps as some of their conclusions say..red blood cells are perhaps not an important way of delivering progesterone to target tissues...no idea how it would be transported in that case!
Anyway..here's some resaerch ..just to confuse matters
This study seems to show that salivary progesterone collected early morning works ..doesn't mention if fasting?
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11532491&dopt=AbstractRelationship between salivary progesterone, 17-hydroxyprogesterone, and cortisol levels throughout the normal menstrual cycle of healthy postmenarcheal girls.
looking at plasma versus saliva tests
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=6638098&dopt=Abstract
Progesterone and estradiol in the saliva and plasma during the menstrual cycle
"There was a highly significant correlation between plasma and salivary progesterone levels throughout the menstrual cycle . Free unbound progesterone as determined by equilibrium dialysis gave a mean +/- SE level of 126.8 +/- 6.9 pg/ml during the proliferative phase and increased significantly to 196.8 +/- 18.8 pg/ml during the secretory phase (p less than 0.001). The corresponding levels in the plasma were 88.5 +/- 11.2 pg/ml, which increased significantly to 332.2 +/- 39.2 pg/ml (p less than 0.001). Free progesterone constituted 53.7% and 41.4% of salivary progesterone during the proliferative and secretory phases, respectively, whereas the corresponding percentages in the plasma were 5.8% and 2.6%. Salivary estradiol levels were 5 to 18 and 8 to 35 pg/ml in the proliferative and secretory phases, respectively, but showed no correlation with plasma estradiol levels."http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?
Salivary progesterone: relation to total and non-protein-bound blood levels.
"The values of non-protein-bound progesterone obtained were significantly and linearly correlated with levels in saliva (r = 0.75, P less than 0.001, d.f. = 34) although the amount of free progesterone in blood was about five times that found in saliva."Now this one is interesting ...different conclusion to heading???
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11809337&dopt=Abstract"We also conclude that the low level of progesterone associated with red cells suggests they are not important in the delivery of progesterone to target tissues."
Salivary, but not serum or urinary levels of progesterone are elevated after topical application of progesterone cream to pre-and postmenopausal women.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11106923&dopt=AbstractCONCLUSIONS: Salivary progesterone measurements confirm that topically applied progesterone is absorbed, despite the lack of change in serum progesterone concentrations. However, at the dose administered, serum progesterone levels do not reach those observed after oral or vaginally delivered progesterone preparations. Higher doses may be required to induce biological responses within the endometrium.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11910616&dopt=Abstract
Effect of sequential transdermal progesterone cream on endometrium, bleeding pattern, and plasma progesterone and salivary progesterone levels in postmenopausal women.
Wren BG, McFarland K, Edwards L, O'Shea P, Sufi S, Gross B, Eden JA.
Sydney Menopause Centre, Royal Hospital for Women, Barker Street, Randwick, New South Wales 2031, Australia.BACKGROUND: Transdermal progesterone is being used in some countries as a purported treatment for menopausal symptoms, either alone or prescribed in conjunction with estrogen, but little information exists regarding the biological activity and effectiveness of this method of delivery of progesterone in protecting the endometrium from excess proliferation. This study was designed to evaluate the use of sequential transdermal progesterone. End-points evaluated included endometrial cellular response and bleeding pattern as well as plasma hormone levels and salivary progesterone estimations. METHOD: Twenty-seven postmenopausal women were treated with continuous transdermal estrogen (28-day cycle) and a cream containing 16, 32 or 64 mg of progesterone in each 4-cm extrusion from a tube of Pro-Feme administered daily in a sequential (days 15-28 of cycle) regimen. Blood and endometrial samples were analyzed for progesterone response prior to therapy, after the first 14 days of unopposed transdermal estrogen and following 14 days of transdermal progesterone. Saliva samples were taken during the last 14 days of the 84-day study, when the final progesterone cream therapy was being applied. RESULTS: Hormone assay indicated that physiological levels of estradiol were achieved, but progesterone levels were insufficient to induce any detectable change in the endometrium. Only one patient experienced bleeding during the study period. Levels of salivary progesterone were so variable as to be considered completely unreliable in determining the potential influence on biological activity. INTERPRETATION: Pro-Feme transdermal progesterone administered in a 16-, 32- or 64-mg daily dose for 14 days in a sequential regimen does not appear to be effective in inducing a secretory change in a proliferative endometrium. Salivary progesterone levels were not of value in managing the therapy of postmenopausal women
....
Jan
poster:tealady
thread:253283
URL: http://www.dr-bob.org/babble/20030823/msgs/254652.html